RNA Isolation from Single Living Cells using AFM

Microrheology of complex systems and living cells using AFM.

Biological cells display viscoelastic mechanical properties that are a key factor in the regulation of cell processes, such as migration, adhesion and deformability. One interesting tool to probe the mechanical properties of living cells and other complex systems is the Atomic Force Microscopy (AFM). Rheological properties of the sample are obtained usually from the force-indentation (F-δ) meas...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

RNA isolation (Total RNA Extraction from High Polysaccharide Plants). Fatemeh Mehrpooyan*

Probing mechanical properties of living cells with AFM

Mechanical properties of cells play a critical role in many essential biological functions including migration, contraction, differentiation and gene expression. Moreover, cells feel and actively respond to adhesive forces and deformations exerted by the adjacent cells and the extracellular matrix. The cell mechanical behavior is increasingly recognized as a key determinant of the normal cell f...

SIDR: simultaneous isolation and parallel sequencing of genomic DNA and total RNA from single cells.

Simultaneous sequencing of the genome and transcriptome at the single-cell level is a powerful tool for characterizing genomic and transcriptomic variation and revealing correlative relationships. However, it remains technically challenging to analyze both the genome and transcriptome in the same cell. Here, we report a novel method for simultaneous isolation of genomic DNA and total RNA (SIDR)...